Table 5.

P-values derived from univariable analysis for variables in relation to infectious agent or group of infectious agents’ positivity. P-values < 0.2 but > 0.05 are shown in italics. Significant P-values ≤ 0.05 are shown in bold

Variable	Mhf PCR	CMhm PCR	CMt PCR	Any hp PCR	Bh PCR	Li PCR	Li serology	Li infection	FeLV serology	FIV serology	Retroviral serology	Hepatozoon spp. PCR	FVBP
Age	0.934	0.014	0.584	0.055	0.152	0.712	0.849	0.560	0.023	0.288	0.760	0.937	0.530
Gender  Male/Female (Ref.)	0.920	0.668	0.709	0.896	0.813	0.833	0.854	0.682	0.202	0.585	0.173	0.448	0.420
Breed  Non-Pedigree/Pedigree (Ref.)	0.250	0.161	0.270	0.069	0.156	0.534	0.424	0.331	0.270	0.051	0.020	0.009	0.020
Housing  Access to outdoors/Indoors only (Ref.)	0.041	0.001	0.590	0.001	0.171	0.269	0.148	0.078	0.075	0.015	0.263	0.121	0.062
Lifestyle  Shelter-feral/Owned (Ref.)	0.086	0.026	0.007	0.042	0.187	0.597	0.146	0.118	0.335	0.001	0.012	0.009	0.021
Habitat  Rural/Urban (Ref.)	0.201	0.169	0.348	0.175	0.650	0.515	0.799	0.933	0.526	0.474	0.946	0.007	0.003
District	0.370	0.195	0.136	0.017	0.416	0.205	0.140	0.414	0.105	0.533	0.341	0.728	0.843
Travel history  Never travelled abroad/Travelled abroad (Ref.)	0.233	0.655	0.915	0.891	0.832	0.534	0.371	0.294	0.262	0.503	0.972	0.028	0.025
Health status  Non-healthy/Healthy (Ref.)	0.139	0.011	0.981	0.011	0.864	0.246	0.453	0.214	0.049	0.010	0.003	0.003	0.008
Vaccination status  Never vaccinated/Vaccinated (Ref.)	0.006	0.960	0.017	0.420	0.445	0.876	0.036	0.063	0.514	0.943	0.861	0.006	0.006
Ectoparasitic prevention status  Never used/Used (Ref.)	0.634	0.226	0.123	0.054	0.349	0.603	0.149	0.072	0.186	0.044	0.429	0.007	0.008
Anaemia  Anaemic/Non-anaemic (Ref.)	0.344	0.110	0.274	0.334	0.482	0.882	0.087	0.133	0.006	0.025	0.002	0.202	0.876

Abbreviations: Mhf Mycoplasma haemofelis, CMhm “Candidatus Mycoplasma haemominutum”, CMt “Candidatus Mycoplasma turicensis”, Any hp positivity in at least one of the following haemoplasma PCRs; Mhf, CMhm and CMt, Bh Bartonella henselae, Li Leishmania infantum confirmed by DNA sequencing following confirmatory quantitative PCR, Li infection positive DNA sequencing for L. infantum following confirmatory qPCR and/or positive L. infantum ELISA, FeLV feline leukaemia virus, FIV feline immunodeficiency virus, Retroviral serology positive for FeLV and/or FIV serology, FVBP positive for at least one of the PCRs for B. henselae, Ehrlichia/Anaplasma spp. and/or Hepatozoon spp., and/or L. infantum infection (i.e. positive DNA sequencing for L. infantum following confirmatory qPCR and/or positive L. infantum ELISA), Ref. reference category

Note: The P-values, χ ² and degrees of freedom from Chi-square analysis are reported in the Additional file 2. Table S1. The P-and Z-values derived from Mann-Whitney U-tests are reported in the Additional file 3. Table S2