FIG. 2.

Phenamil treatment exerts the inhibitory effect on the adipogenic differentiation of hBMSCs by prohibition of PPARγ. To measure their adipogenic differentiation, hBMSCs were cultured with AD supplemented with BMP-2 300 ng/mL, or phenamil 10 μM, or both. (A) Adipogenic gene expression, including PPARγ, Adiponectin, and LPL, was measured by real-time PCR assay at day 3. (B, C) Lipid accumulation was assessed by OR staining at day 14 and AdipoRed assay at day 7. (D) Phenamil prohibits adipogenesis by induction of Trb3 that negatively regulates PPARγ expression as analyzed by western blot assay at day 3. Scale bar = 500 μm. Data presented as mean ± SD (n = 3/group), *p < 0.05 versus AD. AD, adipogenic differentiation; OR, Oil red staining; PPARγ, peroxisome proliferator-activated receptor gamma. Color images available online at www.liebertpub.com/tea