Figure 1. NCF1 loci in PLB-985 cell lines.

(A) NCF1 gene is flanked by two pseudogenes, NCF1B and NCF1C. (B) Differentiators between NCF1 and pseudogenes. PCR primers: Fwd1, Rev1, Rev2. (C) DNA sequence of GT-dinucleotide and 20 nucleotide repeat loci. CRISPR/Cas9-targeted sequence, protospacer adjacent motif (PAM) and BsrG1 site: rectangles; 20-nucleotide repeat: squared brackets. (D) Loss of BsrG1 restriction site in PCR amplified NCF1 (B,C) exon 2 (primers Fwd1 and Rev1 (B)) in PLB-985 NCF1 ΔGT cells. Full-length gel is presented in Supplementary Fig. S6. (E) Percentages of reads from SMRT-seq of PCR products (primers Fwd1 and Rev2 (B)). The absolute number of analyzed SMRT sequencing reads which have passed all filtering criteria is given.