Figure 4. Effects of an HC diet on the expression of phosphorylated AMP-activated protein kinase alpha (p-AMPKα) and phosphorylated acetyl-CoA carboxylase (p-ACC) in the livers of lactating cows.

(A) Western blot analysis of p-AMPKα protein expression in the liver after treatment with an HC diet. (B) Western blot analysis of p-ACC protein expression in the liver after treatment with an HC diet. The experiments were repeated three times. Data are presented as the means ± SEM (n = 5/group). *p < 0.05 indicates statistically significant differences when compared with the control group.