Figure 4.

Sucrose, but not AA, application to the lips results in an increase of cerebral-buccal connective spike activity in preparations from naive snails. (A) Simultaneous recording from both cut cerebral-buccal connectives showing the response of the two connectives to sucrose (0.02 M, 30 sec) applied to the lips. (B) Record from another preparation showing the lack of a cerebral-buccal connective response to AA (0.54 mM, 30 sec) applied to the lips in a naive preparation. (C) Time course of the average changes of spike activity in the cerebral-buccal connective in response to sucrose (n = 5 preparations) and AA (n = 7 preparations) in naive snails (error bars not shown for clarity). The difference in the average frequency change during the 30 sec of stimulus application was statistically significant.