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. 2017 Mar 13;7:44304. doi: 10.1038/srep44304

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(A to E) represent the process of obtaining stable transgenic cotton plants. (A) Sterile cotton seeds were germinated on moistened filter paper in sterile glass plate. (B) Seeds with 1–2 cm length root were moved to glass vessels (5 cm in diameter, 8 cm in height) with MS medium (pH 5.8–6.0) and then grew at 28 °C in darkness. (C) One of each seedling’s cotyledons was removed and the naked shoot apex was injured with a sterilized scalpel. A small sterile absorbent cotton ball with Agrobacterium suspension was placed on the injured shoot apex and vacuum-infiltrated at 0.5 MPa for 10 min at room temperature. (D) Infected seedlings were co-cultivated in an incubator at 28 °C for 2 d in darkness and 2 d under light. (E) Seedlings were transferred to soil and grew in the greenhouse.