Table 2. Analysis of mutations in potential off-target sites in transgenic plants.
sgRNA | No. of putative off-target sites | No. of loci of putative off-target sites | No. of examined loci | No. of plants examined | No. of off-target sites |
---|---|---|---|---|---|
GhCLA1-sgRNA5 | 22 | 36 | 13 | 4 | 0 |
GhVP-sgRNA4 | 19 | 28 | 17 | 3 | 0 |
Only plants containing the verified GhCLA1 or GhVP gene mutations were analyzed for off-target mutations.