Fig. 3.

Detection of Kir2.1Y and Kir2.1 channel functional surface expression. (a) K⁺ channel activity measured by Rb⁺ efflux assayed at the indicated time periods. Results are for 293 cells stably expressing either Kir2.1 (Upper)or Kir2.1Y (Lower) channel after MTSET treatment (□), Rb⁺ efflux of 293 cells stably expressing Kir2.1 channel without MTSET treatment (▪) and mock 293 cells treated with (○) or without (•) MTSET. (b) Flow cytometry analyses of 293 cells stably expressing Kir2.1Y channel with MTSET treatment at different time points (0, 3, and 6 h) before staining with anti-HA antibody to quantify the surface expression of Kir2.1Y. FL, fluorescence intensity in a logarithmic scale. Events refers to the number of cells.