Fig. 4.

Temperature-dependent activity recovery of Kir2.1Y channel determined by Rb⁺ efflux. (a–c) Activity recovery of Kir2.1Y stable cell line after MTSET treatment (□), Rb⁺ efflux of Kir2.1Y stable cell line without MTSET treatment (▪), and Rb⁺ efflux of mock 293 cells after MTSET treatment (○)or without MTSET treatment (•). The recovery experiments were carried out at 15°C (a), 22°C (b), and 30°C (c). All measurements were performed in triplicate as described in Methods. (d) Normalized recovery rates from 0 to 5 h at 15°C (▪), 22°C (□), 30°C (▴), and 37°C (⋄) (from Fig. 3a). The Rb⁺ efflux of Kir2.1Y without MTSET treatment was set to 100%.