Fig. 5.

BFA effect on activity recovery of Kir2.1Y channel. (a) We incubated 293 cells stably expressing Kir2.1Y (hatched bars) and mock 293 cells (open bars) with 1 μM BFA at 37°C for 3 h after a 5-min MTSET treatment or without MTSET treatment. In control samples, neither MTSET nor BFA were added. Each column is the average of two samples. (b) Dose-dependent effect by BFA treatment. Results show 293 cells stably expressing Kir2.1Y that were incubated with BFA only (▪), cells stably expressing Kir2.1Y that were treated with MTSET before incubation with BFA (□), mock 293 cells incubated with BFA only (•), and mock 293 cells treated with MTSET before incubation with BFA (○). All experiments were performed in duplicate with 3-h incubation. The Rb⁺ efflux (%) is plotted against different concentrations of BFA at 37°C before Rb⁺ efflux assay.