Table 1. Efficiency of transplant with mitosomes containing APSK-HA into recipient strains.
| Recipient | Time* | N# | Cells |
Mitosomes |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of Injected | No. of Examined | No. of Detected | Total Rate | Mean | SD | SE | No. of Detected (merged) | Max. No. per cell | Density$ | |||
| HM-1: IMSS | 30 min | 4 | 120 | 34 | 8 | 0.24 | 0.27 | 0.23 | 0.11 | 30 | 15 | 0.88 |
| AS-Myc | 30 min | 10 | 371 | 76 | 17 | 0.22 | 0.25 | 0.18 | 0.06 | 22 (4) | 5 | 0.29 |
| 60 min | 8 | 313 | 58 | 10 | 0.17 | 0.09 | 0.12 | 0.04 | 32 (9) | 11 | 0.55 | |
| 120 min | 6 | 247 | 67 | 1 | 0.02 | 0.01 | 0.03 | 0.01 | 1 (0) | 1 | 0.02 | |
| G3 | 30 min | 3 | 121 | 24 | 13 | 0.54 | 0.58 | 0.24 | 0.14 | 42 | 6 | 1.75 |
Transplant efficiency is shown as the percentage of cells with donor mitosomes per number of examined cells. The number of incorporated organelles was estimated to be 2.8-13 per cell.
*Time indicates the processing time for microinjection and post-incubation until fixation.
#No. of Exp. (number of experiments) indicates the number of independent mitosome isolations.
$Number of detected mitosomes per number of examined cells.