FIG 2 .

Phylogenetic tree of 163 concatenated virulence factors shared by all V. anguillarum strains. The phylogenetic tree was constructed based on the maximum likelihood algorithm, using a concatenated alignment of 163 amino acid sequences inferred from putative virulence factors identified in V. anguillarum strain 775 (20) (Table S2). Bootstrap values <80% were removed from the tree. The horizontal bar at the base of the figure represents 0.01 substitutions per amino acid site. The virulence ranking of the strains is based on three fish larva models (31). HV, high virulence; LV, low virulence; MV, medium virulence.