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. Author manuscript; available in PMC: 2018 Jan 20.
Published in final edited form as: ACS Chem Biol. 2016 Nov 29;12(1):114–123. doi: 10.1021/acschembio.6b00732

Figure 4.

Figure 4

Substrate selectivity of wild-type and mutant DEBS3. (A) Scheme illustrating a competition assay to determine extender unit selectivity of DEBS3. Asterisk indicates domain location of mutations. (B) Extracted ion chromatograms of DEBS3-catalyzed chain extension reactions (7 [M-H2O+H]+ m/z = 279.1955; 8 [M-H2O+H]+ m/z = 303.1955; 9 [M-H2O+H]+ m/z = 337.2366; 10 [M-H2O+H]+ m/z = 361.2373). See Experimental Section for a complete description of product identification and quantification. (C) Product distribution of DEBS3-catalyzed chain reactions (values are mean ± SD; n = 3).