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. Author manuscript; available in PMC: 2018 Jan 20.
Published in final edited form as: ACS Chem Biol. 2016 Nov 29;12(1):114–123. doi: 10.1021/acschembio.6b00732

Figure 5.

Figure 5

Extender unit promiscuity of wild-type and mutant DEBS3. (A) Scheme illustrating the competition assay to determine specificity of DEBS3 towards each extender unit. Asterisk indicates location of mutations. (B) Fraction of each 10-DML analogue 12a–d as a percentage of the total products (sum ion counts for 7+9+12+13) of DEBS3-catalyzed chain reactions using each extender unit 11a–d (values are mean ± SD; n = 3). See Experimental Section for complete description of product identification and quantification.