FIG 2 .

Antigenic profiles of N332 epitope scaffolds and nanoparticles. (A) Antigenic evaluation of 14 epitope scaffolds (left) and nanoparticles (right) presenting the N332 supersite against three representative N332-dependent bNAbs—PGT121, PGT128, and PGT135. The OD₄₅₀ value at the highest antigen concentration (50 μg/ml) is shown, with the ELISA curves presented in Fig. S4. (B to D) Octet binding of monomeric N332 scaffolds and their respective nanoparticles to a panel of 5 N332-dependent bNAbs is shown for 1GUT_A_ES and 1GUT_A_ES-FR (B), 1KIG_L_ES-2 and 1KIG_L_ES-2-FR (C), and 2CCQ_A_ES and 2CCQ_A_ES-FR (D). Sensorgrams were obtained from an Octet RED96 instrument using a titration series of six starting at the maximum of 500 nM for monomeric epitope scaffolds and 50 nM for nanoparticles, respectively.