FIG 3 .

Impact of additional S10 copies in V. cholerae. (a) Several S10 copies (orange arrows) were inserted within the S10Tnp+166Δ(aph,cat) genome. Their insertion sites, drawn as black arrows and the coordinates (in kilobase pairs from the original location), are shown within V. cholerae chromosomes. (b) The S10 ploidy effect on growth rate was quantified by averaging obtained μ in at least 3 independent experiments, with 3 or more biological replicates, for each mutant strain and normalizing it to the μ of the parental strain. Results are expressed as percentage of the variation (% μ) with respect to parental strains. (c) Competition experiments done under fast-growth conditions. For panels b and c, data are shown using box-and-whisker plots. Statistical significance was analyzed by a nonparametric test, and the Dunn test was done to compare the mean values obtained for each strain. ****, P < 0.0001.