Table 1. Table of significant features for uninfected (Control) Ixodes scapularis nymphs versus nymphs infected with the Lyme disease spirochetes Borrelia burgdorferi (Bb) or Borrelia mayonii (Bm).
| Annotation |
p-value |
Prefeed-Fold Change |
Day 1-Fold Change |
Day 2-Fold Change |
Day 3-Fold Change |
Day 4-Fold Change |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ID | Functional Class | KEGG ID | MSI Conf. | trt | day | trt:day | Control /Bb | Control /Bm | Control /Bb | Control /Bm | Control /Bb | Control /Bm | Control/Bb | Control/Bm | Control/Bb | Control/Bm |
| Adenosine, alpha- (4TMS) MP | Nucleic Acid | C00212 | 2 | 0.0071 | 0.0014 | 0.0656 | 2.5 | 3.6 | 1.2 | 0.9 | 5.8 | 7.7 | 1.5 | 1.6 | 0.7 | 2.6 |
| Adenosine-5-monophosphate (5TMS) | Nucleic Acid | C00020 | 2 | 0.0219 | <0.0001 | 0.1554 | 0.8 | 0.8 | 1.3 | 0.9 | 0.8 | 0.8 | 1.0 | 1.7 | 1.0 | 3.2 |
| Alanine (2TMS) | Amino Acids | C00041 | 2 | 0.0048 | <0.0001 | 0.0193 | 1.1 | 1.0 | 1.4 | 0.5 | 2.3 | 1.9 | 1.3 | 1.1 | 1.1 | 2.3 |
| Allantoin (4TMS) | Ureide | C02350 | 2 | 0.1195 | 0.0001 | 0.5881 | 1.0 | 1.0 | 1.4 | 0.7 | 2.2 | 1.8 | 1.3 | 1.5 | 0.9 | 2.0 |
| Benzoic acid, (1TMS) | Co-factor | C00180 | 1 | 0.0027 | 0.0003 | 0.0041 | 1.6 | 0.5 | 3.6 | 1.0 | 1.2 | 2.0 | 0.9 | 0.7 | 1.5 | 3.7 |
| Campesterol (1TMS) | Lipid | C01789 | 2 | 0.0281 | <0.0001 | 0.0017 | 0.8 | 0.8 | 1.2 | 1.3 | 0.9 | 1.5 | 0.7 | 0.6 | 0.8 | 2.8 |
| Carbodiimide (2TMS) | Xenobiotic | C01566 | 2 | 0.0005 | 0.0002 | 0.0106 | 2.1 | 1.8 | 0.3 | 1.7 | 0.7 | 3.7 | 1.5 | 1.5 | 1.2 | 2.2 |
| Cholesterol (1TMS) | Lipid | C00187 | 2 | 0.0004 | <0.0001 | 0.0022 | 2.2 | 1.4 | 2.1 | 1.2 | 1.7 | 2.3 | 0.8 | 1.0 | 1.0 | 2.6 |
| Cystathionine (4TMS) | Amino Acids | C02291 | 2 | 0.0010 | <0.0001 | 0.0013 | 0.9 | 0.8 | 0.7 | 0.9 | 0.9 | 1.7 | 1.4 | 1.2 | 1.2 | 7.2 |
| Dodecanoic acid (1TMS) | Lipid | C02679 | 2 | <0.0001 | 0.0003 | 0.0001 | 1.8 | 1.0 | 2.3 | 1.3 | 1.3 | 1.9 | 1.2 | 1.2 | 1.1 | 3.2 |
| Ethanolamine (3TMS) | Amine | C00189 | 2 | 0.0023 | <0.0001 | 0.0146 | 1.6 | 1.3 | 1.4 | 1.8 | 1.5 | 1.6 | 1.6 | 2.5 | 1.0 | 5.7 |
| Ethanolaminephosphate (4TMS) | Lipid | C00346 | 2 | 0.0083 | <0.0001 | 0.0476 | 1.7 | 0.8 | 1.5 | 0.8 | 2.6 | 2.1 | 1.2 | 1.2 | 1.0 | 4.2 |
| Galactose (1MEOX) (5TMS) BP.1 | Carbohydrates | C00124 | 2 | 0.0265 | 0.0001 | 0.0305 | 1.2 | 1.6 | 1.6 | 1.0 | 1.6 | 1.5 | 1.6 | 1.0 | 1.7 | 11.2 |
| Gentiobiose (1MEOX) (8TMS) BP | Carbohydrates (Xeno) | C08240 | 2 | 0.0080 | 0.0001 | 0.0155 | 1.1 | 0.9 | 1.2 | 1.5 | 4.7 | 5.1 | 6.5 | 3.1 | 0.9 | 2.9 |
| Glutamic acid (3TMS) | Amino Acids | C00025 | 2 | <0.0001 | <0.0001 | 0.0001 | 1.5 | 0.7 | 1.1 | 0.3 | 2.1 | 1.7 | 1.2 | 1.3 | 1.0 | 2.4 |
| Glutamine, DL- (3TMS) | Amino Acids | C00064 | 2 | 0.1856 | <0.0001 | 0.0249 | 4.7 | 0.9 | 1.8 | 0.7 | 2.4 | 1.8 | 3.0 | 1.0 | 0.6 | 1.7 |
| Glycerol (3TMS) | Carbohydrates | C00116 | 2 | 0.0004 | <0.0001 | 0.0066 | 1.5 | 1.5 | 1.2 | 1.1 | 1.7 | 2.1 | 1.4 | 1.3 | 1.4 | 3.0 |
| Glycerol-3-phosphate (4TMS) | Lipid | C00093 | 2 | 0.0004 | <0.0001 | 0.0025 | 1.7 | 0.9 | 4.2 | 1.9 | 3.0 | 2.6 | 1.2 | 1.3 | 0.8 | 2.8 |
| Glycine (3TMS) | Amino Acids | C00037 | 2 | <0.0001 | <0.0001 | 0.0009 | 1.6 | 1.4 | 1.4 | 0.5 | 2.3 | 2.0 | 1.4 | 1.1 | 1.2 | 2.3 |
| Guanine (3TMS) | Nucleic Acid | C00242 | 2 | 0.0214 | 0.0001 | 0.0797 | 2.1 | 1.4 | 2.1 | 0.8 | 2.9 | 5.0 | 1.4 | 2.3 | 0.6 | 3.0 |
| Guanosine (5TMS) | Nucleic Acid | C00387 | 2 | 0.0025 | 0.0003 | 0.0856 | 4.2 | 4.2 | 1.8 | 0.7 | 5.2 | 7.3 | 1.6 | 2.0 | 0.7 | 3.2 |
| Hexadecanoic acid (1TMS) | Lipid | C00249 | 2 | <0.0001 | <0.0001 | <0.0001 | 1.5 | 0.3 | 1.0 | 0.3 | 1.1 | 0.3 | 0.9 | 0.4 | 1.0 | 1.1 |
| Hypoxanthine (2TMS) | Nucleic Acid | C00262 | 2 | 0.1108 | 0.0002 | 0.5390 | 1.0 | 1.0 | 1.5 | 0.8 | 8.4 | 8.4 | 1.1 | 1.2 | 1.0 | 2.4 |
| Inosine (4TMS) | Nucleic Acid | C00294 | 2 | 0.0595 | <0.0001 | 0.3658 | 74.9 | 4.5 | 39.1 | 29.3 | 0.0 | 0.0 | 0.5 | 1.6 | 1.8 | 17.2 |
| Isoleucine (2TMS) | Amino Acids | C00407 | 2 | 0.0045 | <0.0001 | 0.0211 | 1.5 | 0.4 | 2.1 | 0.2 | 3.4 | 2.6 | 2.1 | 1.2 | 1.2 | 3.9 |
| Leucine (1TMS) | Amino Acids | C00123 | 2 | 0.0017 | <0.0001 | 0.0134 | 1.5 | 1.1 | 1.9 | 0.5 | 3.7 | 4.2 | 1.8 | 1.1 | 1.3 | 4.1 |
| Lysine (4TMS) | Amino Acids | C00047 | 2 | 0.0009 | <0.0001 | 0.0011 | 1.6 | 1.5 | 0.9 | 0.4 | 2.4 | 1.5 | 1.5 | 1.5 | 1.0 | 3.2 |
| Maltose (1MEOX) (8TMS) MP | Carbohydrates | C00208 | 2 | 0.0036 | <0.0001 | 0.0071 | 1.5 | 1.9 | 1.3 | 1.6 | 4.8 | 6.7 | 5.5 | 3.3 | 0.8 | 2.9 |
| myo-Inositol-1-phosphate (7TMS) | Co-factor | C01177 | 2 | 0.0016 | <0.0001 | 0.0184 | 1.5 | 1.5 | 1.1 | 0.8 | 2.6 | 2.1 | 1.0 | 1.1 | 1.0 | 2.3 |
| NA114002 (classified unknown) | unknown | unknown | 2 | 0.0005 | <0.0001 | 0.0012 | 1.3 | 0.9 | 1.1 | 1.0 | 1.2 | 1.2 | 1.1 | 1.0 | 1.0 | 1.1 |
| Octadecenoic acid, 9-(Z)- (1TMS) | Lipid | C00712 | 2 | <0.0001 | <0.0001 | 0.0017 | 1.4 | 0.7 | 1.9 | 1.4 | 1.7 | 2.7 | 1.3 | 1.2 | 1.2 | 3.6 |
| Ornithine (4TMS) | Amino Acids | C00077 | 2 | 0.0045 | <0.0001 | 0.0072 | 2.5 | 3.2 | 1.5 | 0.7 | 1.7 | 1.5 | 1.5 | 1.1 | 1.0 | 3.8 |
| Pantothenic acid, D- (3TMS) | Vitamins | C00864 | 2 | <0.0001 | <0.0001 | 0.0002 | 1.1 | 0.8 | 1.6 | 0.8 | 3.6 | 2.9 | 2.1 | 1.8 | 1.1 | 3.0 |
| Phenylalanine (2TMS) | Amino Acids | C00079 | 2 | 0.0003 | <0.0001 | 0.0017 | 1.8 | 0.9 | 1.9 | 0.4 | 1.9 | 2.5 | 2.2 | 1.4 | 1.4 | 4.7 |
| Phosphoric acid (3TMS) | Co-factor | C00009 | 2 | 0.0004 | <0.0001 | 0.0015 | 1.0 | 1.1 | 0.9 | 1.3 | 1.5 | 1.7 | 1.0 | 1.0 | 1.0 | 2.0 |
| Phosphoric acid monomethyl ester (2TMS) | Xenobiotic | C19998 | 2 | 0.0042 | <0.0001 | 0.0095 | 1.0 | 1.2 | 1.0 | 0.7 | 4.0 | 2.4 | 0.6 | 1.2 | 0.9 | 3.3 |
| Proline (1TMS) | Amino Acids | C00148 | 2 | <0.0001 | <0.0001 | <0.0001 | 1.1 | 1.0 | 1.0 | 1.0 | 2.3 | 1.6 | 1.1 | 0.8 | 1.1 | 4.7 |
| Pyroglutamic acid (2TMS) | Amino Acids | C01879 | 2 | <0.0001 | <0.0001 | 0.0006 | 2.6 | 1.2 | 1.4 | 0.8 | 2.0 | 1.7 | 1.3 | 1.6 | 0.9 | 2.8 |
| Serine (3TMS) | Amino Acids | C00065 | 2 | 0.0012 | <0.0001 | 0.0052 | 1.8 | 1.0 | 1.1 | 0.5 | 1.9 | 1.9 | 1.7 | 1.4 | 1.0 | 3.3 |
| Sitosterol, beta- (1TMS) | Lipid | C01753 | 2 | 0.0874 | <0.0001 | 0.0078 | 0.9 | 1.4 | 1.0 | 1.5 | 0.5 | 1.5 | 0.5 | 0.4 | 0.8 | 3.3 |
| Sucrose (8TMS) | Carbohydrates | C00089 | 2 | 0.0010 | 0.2407 | 0.0043 | 2.2 | 1.0 | 1.1 | 0.1 | 1.2 | 0.1 | 1.7 | 1.2 | 1.0 | 1.4 |
| Threonine (3TMS) | Amino Acids | C00188 | 2 | 0.0024 | <0.0001 | 0.0236 | 1.5 | 1.1 | 1.2 | 0.5 | 2.0 | 2.4 | 1.9 | 1.1 | 1.3 | 3.2 |
| Tryptophan (3TMS) | Amino Acids | C00078 | 2 | 0.1798 | <0.0001 | 0.0103 | 1.3 | 0.5 | 1.0 | 0.6 | 1.5 | 0.8 | 1.0 | 0.9 | 0.9 | 1.6 |
| Tyrosine (3TMS) | Amino Acids | C00082 | 2 | 0.0028 | <0.0001 | 0.0136 | 1.2 | 1.2 | 1.6 | 0.5 | 1.6 | 1.6 | 1.5 | 1.4 | 1.0 | 3.3 |
| Uracil (2TMS) | Nucleic Acid | C00106 | 2 | 0.0021 | <0.0001 | 0.0471 | 1.2 | 0.8 | 0.5 | 0.5 | 2.4 | 2.0 | 1.1 | 1.1 | 1.2 | 2.1 |
| Urea (2TMS) | Amino Acids | C00086 | 2 | 0.0045 | <0.0001 | 0.0366 | 1.1 | 0.5 | 2.3 | 0.6 | 2.9 | 2.9 | 1.0 | 1.1 | 1.3 | 2.8 |
| Valine (2TMS) | Amino Acids | C00183 | 2 | <0.0001 | <0.0001 | 0.0002 | 1.4 | 0.7 | 1.2 | 0.4 | 3.1 | 2.3 | 2.1 | 1.4 | 1.1 | 3.8 |
| Xanthine (3TMS) | Nucleic Acid | C00385 | 2 | 0.0001 | <0.0001 | 0.0001 | 1.1 | 0.8 | 1.0 | 0.6 | 6.5 | 4.4 | 0.8 | 1.2 | 0.7 | 0.7 |
| Xanthosine (5TMS) | Nucleic Acid | C01762 | 2 | 0.2085 | <0.0001 | 0.0340 | 2.1 | 0.9 | 1.3 | 0.2 | 7.0 | 1.3 | 1.4 | 1.4 | 0.4 | 1.6 |
A. KEGG: Kyoto Encyclopedia of Genes and Genomes; B. MSI Conf. Metabolomics Standards Initiative confidence of annotation; C. p-value significance by ANOVA analysis of treatment (trt: Borrelia infection status), time, and treatment:time (trt:time). D. Fold change determined by dividing relative abundance of selected metabolite in control ticks by relative abundance of metabolite in the Borrelia infected groups.