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. 2017 Mar 13;7:44388. doi: 10.1038/srep44388

Figure 5. FK506 increases DNase activity of SF70 by ~100-fold and in a reversible manner.

Figure 5

(A) Closed and open symbols indicate that FK506 or DMSO vehicle, respectively, was added to a 1:1 mixture of SF70-NFM and SF70-CFM at time zero. Circles, squares, and triangles designate total enzyme concentrations of 20 μM, 10 μM, and 4 μM, respectively. (B) Reversibility is demonstrated by adding FK506 to a mixture of SF70-NFM and SF70-CFM (10 μM) as in panel A, waiting until full enzymatic activity is reached (arrow), and then adding a 10-fold excess of free FKBP to compete off FK506.