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. 2017 Feb 16;11(2):93–98. doi: 10.22074/ijfs.2017.5049

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Fig.3 — Confirmation of presence of nuclei in cloned embryos. During the SCNT procdure, some embryos may become fragmented and providing blastomeres without nuclei. Therefore, combined staining with nuclear dye (H33342) and DCHFDA was carried out to investigate this phenomenon. As show, except for one blastomere without nucleus (arrow), all the other blastomeres with high intensity for DCHFDA had nuclei. Bar represents 50 μm.

SCNT; Somatic cell nuclear transfer and DCFHDA; 2, 7-dichloro dihydroflourescein diacetate.