Fig. 5.

No evidence for a HP-specific gene-expression profile. (A) Log-scale plot of normalized and averaged expression for indicated populations versus naïve expression. Genes up-regulated at day 6 of HP compared to naïve T cells (>1.3-fold over naïve P value < 0.01) and also compared effector and memory cells (>1.3-fold) are highlighted in black (219 genes). (B) Fold-change (log₂) versus P value plotted for cells transferred into an unirradiated host (no Rad) compared to naïve or a random data set compared to naïve. Highlighted genes are those up-regulated in HP as described above. (C) Relative expression to naïve T cells by effector (one sample), memory (two samples), no Rad (one sample), and HP (two samples) as determined by quantitative PCR for nine candidate HP-specific genes. Data are representative of at least three unique samples and three different PCR experiments.