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R17 phage replication is disrupted in tsf mutants. (A) The locations of EF-Ts Ala202 and Arg219 are shown in the context of the Qβ replicase complex (PDB ID code 3MMP). (B) M13 and R17 phage lysates were serial diluted and spotted onto lawns of E. coli MG1655 F´ cells that carry tsf+, tsf(A202E), tsf(R219P), and tsf(∆coil) alleles. The numbers of phage delivered per spot are indicated.
