Table S2.
Plasmids
| Plasmid | Description | Source |
| pRK6 | pBAD33::gfp, AmpR CmR | (46) |
| pRK793 | Expresses His6-tagged TEV protease, AmpR | Addgene |
| pWEB::TNC | Cosmid cloning vector, AmpR CmR | Epicentre |
| pRE112 | Allelic exchange vector, CmR | (39) |
| pZS21-bamA+ | pZS21 derivative that expresses E. coli bamA, KanR | (47) |
| pCH2262 | Constitutive expression of chimeric cdiAEC93-CTO32:H37 and cdiIO32:H37, CmR | This study |
| pTrc99KX | Derivative of pTrc99a that contains a 5′-KpnI restriction site and 3′-SpeI and XhoI sites, AmpR | (12) |
| pCH6243 | pET21::his6-cdiA-CT/cdiIUPEC536, AmpR | (17) |
| pCH9305 | Constitutive expression of chimeric cdiAEC93-CTo11EC869 and cdiIo11EC869, CmR | (15) |
| pCH10163 | Cosmid pCdiA-CT/pheS* that carries a kan-pheS* cassette in place of the E. coli EC93 cdiA-CT/cdiI coding sequence. Used for allelic exchange and counter selection, CmR KanR | (15) |
| pCH10068 | pSH21P::trxA-TEV-rhs-CT(H208A)orphan-rhsIorphan, orphan rhs-CT/rhsI module fused to His6-tagged thioredoxin gene through TEV cleavage site, AmpR | (48) |
| pCH10256 | pTrc99KX::cdiIEC869, AmpR | This study |
| pCH10445 | Constitutive expression of chimeric cdiAEC93-CTECL and cdiIECL, CmR | (12) |
| pCH10525 | Constitutive expression of chimeric cdiAEC93-CTEC869 and cdiIEC869, CmR | (16) |
| pCH10673 | Constitutive expression of chimeric cdiAEC93-CTEC536 and cdiIEC536, CmR | (16) |
| pCH11434 | Constitutive expression of chimeric cdiAEC93-CTNC101 and cdiINC101, CmR | (5) |
| pCH11435 | Constitutive expression of chimeric cdiAEC93-CT96.154 and cdiI96.154, CmR | This study |
| pDAL660Δ1–39 | Constitutive expression of cdiAEC93 and cdiIEC93, CmR | (9) |
| pDAL1028 | pREG153 carrying sfsA – dxr of the E. coli MG1655 chromosome, AmpR | This study |
| pDAL1029 | pRE112::tsf(A202E), CmR | This study |
| pDAL1030 | pRE112::tsf(R219P), CmR | This study |
| pDAL1031 | pRE112::tsf(∆coil), CmR | This study |
| pDAL1032 | pRE112::∆wzb, CmR | This study |
| pDAL1033 | pSH21::trxA-TEV-CTEC869-cdiIEC869, AmpR | This study |
| pDAL1034 | pSH21::trxA-TEV-tsf, AmpR | This study |
| pDAL1035 | pSH21::trxA-TEV-tufA, AmpR | This study |
| pDAL1036 | pSH21::trxA-TEV-tsf(A202E), AmpR | This study |
| pDAL1037 | pSH21::trxA-TEV-tsf(∆coil), AmpR | This study |
| pDAL1038 | pET21b::cdiIEC869, AmpR | This study |
| pDAL1039 | pET21::his6-DUF-cdiA-CTEC869-cdiIEC869, AmpR | This study |
AmpR, ampicillin resistance; CmR, chloramphenicol resistance; KanR, kanamycin resistance.