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MWCNT (10 µg) aspiration acutely reduces BBB integrity and induces GFAP expression on astrocytes. (A and B) Fluorescein staining (green) is not apparent in control-treated mouse brains (A), but is clearly adjacent to vascular structures (red; PECAM) in MWCNT-treated mice (B). (C–H) Enhanced GFAP staining (red) in the cerebellum (C–E) and hippocampus (F–H) of control and MWCNT-treated mice, indicative of astrocyte activation. *P <0.05, Student’s t test.
