Fig. 4.

Both WT and phosphonull melanopsin rescue the masking deficit in MKO. A comparison of wheel-running activities recorded in 10-min bouts between the day before and day of a 3-h light pulse in MKO (A), AAV2 (melanopsin) (B), and AAV2 (phosphonull) (C) is shown. A 3-h light pulse initiated at Zeitgeber time 14 (ZT14) is indicated by the red box. (D) Comparison of the normalized activities of the three groups represented in 20-min bins from ZT13 to ZT18. Wheel revolutions were normalized to the highest bout, which occurs outside of the window of time shown here. Statistical analyses conducted in A–C for before and after comparisons were linear mixed-effect models comparing activities on the day before and day of a 3-h light pulse in MKO (P = 0.042), AAV2 (melanopsin; P = 0.009), and AAV2 (phosphonull; P = 0.045). Comparison of the three groups in D was conducted by two-way ANOVA with multiple comparisons (**P ≤ 0.01, ***P ≤ 0.001).