Fig. S3.

Viral infectivity and colocalization of mRuby and melanopsin in the retina. Four weeks after intravitreal injection with either AAV2/(mRuby-P2A-melanopsin-FLAG) (A and C) or AAV2/(mRuby-P2A-CPΦ-melanopsin-FLAG) (B, D, and E), the retinas were harvested and immunostained with rabbit antimelanopsin antibody or imaged directly without immunostaining. Fluorescent images from five different retinas depict antimelanopsin staining on the plasma membrane of ipRGCs (A, B, D, and E) and mRuby in the cytoplasm (A–E). All infected cells in the field showed colocalization of mRuby and melanopsin in the same cells.