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. 2017 Feb 22;114(10):2556–2561. doi: 10.1073/pnas.1611771114

Fig. 4.

Fig. 4.

Retention of cartilage phenotype. (A) The IC group showed type I collagen deposition, loss of type II collagen, faint lubricin expression in upper and lower aspects, and extensive osteopontin expression. The CH group showed homogeneous type II collagen deposition, superficial-zone lubricin expression, and deep-zone osteopontin expression. PDGF-BB staining revealed preosteoclasts/osteoclasts invading IC discs but not CH discs. (Scale bar, 100 μm.) (B) The GAG content of the CH group recovered whereas that of the IC group deteriorated postimplantation. COL contents were similar. (C) Expression of cartilage maintenance (SOX9, ACAN, COL2A1, COL9A1, COL11A1) and chondrocyte terminal differentiation (COL10A1, RUNX2) markers persisted in the CH group but not the IC group postimplantation. All gene expression (2−∆Ct) is normalized to GAPDH. Mean ± SEM; *P < 0.05 and **P < 0.01.