Fig. 4.

RDFs characterizing the solution around the ELP in pure water (black) and 0.55 M solutions of TMAO (red), betaine (green), and glycine (blue). The RDFs in A were calculated using all atoms from the ELP construct, the cosolvents, and water. B and C characterize the density of hydrogen-bonding groups around the ELP backbone NH and CO sites, respectively. B considered distances from the nitrogen atoms of four ELP valine and glycine residues to the oxygen atoms of the cosolvents and water. C considered distances from the carbonyl oxygen atoms of five ELP residues to the nitrogen atom of glycine, the methyl carbon atoms of betaine and TMAO, or the oxygen atom of water.