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The N- and C-terminal domains of P4 mediate association with NBR1. GFP-tagged N- and C-terminal domains as well as the middle domain of P4 were coexpressed with RFP-tagged NBR1. Imaging was carried out 2 d after Agrobacterium-mediated transformation of N. benthamiana leaves. P4 fragments correspond to those illustrated in Fig. 3F. (Scale bars, 10 μm.) BF, bright field.
