Fig. 2.

Overview of Nav-LBT constructs. (A) Sequence alignments of all Nav-LBT constructs. All clones have the LBT inserted at the top of S4 and three consecutive glycines inserted before the LBT. The nomenclature is as follows: Dx-3GLBT-R(−y), where “x” indicates the Nav domain number, in roman numerals, and “y” is the number of amino acids counting back from the first S4 charge of the domain. Where an original glycine exists in the insertion site of 3G before LBT, only two glycines were inserted. Sequences highlighted in yellow indicate putative transmembrane segments. (B) Normalized conductance as a function of voltage (G-V) for all Nav-LBTs. Gray squares correspond to wild-type (WT) Nav1.4. Circles indicate Dx-3GLBT-R(−5) in blue, Dx-3GLBT-R(−6) in red, and Dx-3GLBT-R(−7) in green. Error bars are SEMs.