Skip to main content
. 2017 Feb 15;114(10):E1857–E1865. doi: 10.1073/pnas.1700453114

Fig. 6.

Fig. 6.

LRET under voltage clamp using KIIIA-Bodipy: Voltage-dependent changes in distance for DI and DIV are shown. A representative SE signal in DI-3GLBT-R(−6) (A) and a representative SE signal in DIV-3GLBT-R(−6) (B) in different states are shown. The SE signal in the slow inactivated state recorded first is shown in orange, the SE signal in the resting state is shown in red, and the second recorded SE signal in the slow inactivated state is shown in green. (C) D signal in DI-3GLBT-R(−5) showed a voltage-dependent change. The D signal obtained in the first and second slow inactivated state recordings are shown as black and blue traces, respectively; the resting state D signal is the red trace. (D) Pulse-laser protocols used to induce and record from the slow inactivated and resting states are shown. (E) Distances between KIIIA-Bodipy and DI-LBTs or DIV-LBTs showed significant voltage-dependent changes. Data from Dx-3GLBT-R(−5) clones are shown in blue, data from Dx-3GLBT-R(−6) are shown in red, and data from Dx-3GLBT-R(−7) are shown in green. Error bars represent SEMs. Asterisks indicate significant distance changes (*P < 0.05, unpaired t test).