Table 2.

LRET time constants and estimated distances from intact, unclamped oocytes with GIIIA-Bodipy

GIIIA-Bodipy (n)	τD, ms	τSE, ms	Distance, Å
D1-3GLBT-R(−5) (6)	2.42 ± 0.03	1.08 ± 0.07	39.7 ± 0.7
D1-3GLBT-R(−6) (7)	2.48 ± 0.02	1.2 ± 0.06	40.8 ± 0.6
D1-3GLBT-R(−7) (6)	2.44 ± 0.05	0.97 ± 0.03	38.5 ± 0.3
D2-3GLBT-R(−5) (6)	2.53 ± 0.01	1.25 ± 0.02	41.0 ± 0.2
D2-3GLBT-R(−6) (4)	2.42 ± 0.06	0.93 ± 0.04	38.0 ± 0.4
D2-3GLBT-R(−7) (6)	2.40 ± 0.03	1.02 ± 0.03	39.2 ± 0.4
D3-3GLBT-R(−5) (6)	2.44 ± 0.02	1.45 ± 0.03	43.9 ± 0.2
D3-3GLBT-R(−6) (8)	2.48 ± 0.03	1.49 ± 0.03	44.2 ± 0.4
D3-3GLBT-R(−7) (8)	2.41 ± 0.02	1.44 ± 0.03	44.1 ± 0.5
D4-3GLBT-R(−5) (7)	2.37 ± 0.02	1.01 ± 0.02	39.2 ± 0.2
D4-3GLBT-R(−6) (7)	2.49 ± 0.02	0.98 ± 0.02	38.4 ± 0.2
D4-3GLBT-R(−7) (9)	2.43 ± 0.02	1.01 ± 0.02	38.9 ± 0.2

The number of determinations (n), for each channel construct, is shown in the first column; τ_D indicates the value of the time constant of donor decay in the absence of acceptor; and τ_SE is the decay time constant of the sensitized emission.