Table 3.

LRET time constants and estimated distances from intact, unclamped oocytes with Ts1-Bodipy

Ts1-Bodipy (n)	τD, ms	τSE, ms	Distance, Å
D1-3GLBT-R(−5) (5)	2.42 ± 0.04	1.41 ± 0.002	44.2 ± 0.3
D3-3GLBT-R(−5) (6)	2.35 ± 0.02	1.91 ± 0.04	53.9 ± 1.2
D4-3GLBT-R(−5) (4)	2.38 ± 0.01	2.20 ± 0.02 (τDA)	63.7 ± 1.8

The number of determinations (n), for each channel construct, is shown in the first column; τ_D is the time constant of the donor only signal; and τ_DA, in parenthesis, is the time constant of donor decay in the presence of acceptor instead of the time constant obtained from the sensitized emission, τ_SE.