Fig. S7.

Proteins associated with β-actin 3′-UTR by smFISH-IF. Representative smFISH-IF images in dissociated hippocampal neurons from MBS mice detected by smFISH for β-actin mRNA (MBS FISH probes; red) combined with IF for the indicated proteins (green): (A) YBOX1, (B) Sam68, (C) Dhx9, (D) hnRNPU, (E) hnRNPAB, and (F) hnRNPE2. Yellow arrowheads show sites of molecular interaction as defined by box A in Fig. 2 (probability of chance association < 0.1 and optimal distance = 69 nm); white arrowheads show nonassociated molecules as defined by box B in Fig. 2 (distances between optimal distance and 250 nm). MAP2 is shown in blue as a dendrite marker. Images are representative of (A, D, and E) two and (B, C, and F) three independent experiments, with over 15–20 dendrites observed in each experiment. (Scale bars: 5 μm.)