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. 2017 Jan 30;114(10):E2053–E2062. doi: 10.1073/pnas.1620973114

Fig. 5.

Fig. 5.

HopBA1 interacts with RBA1 and enhances RBA1 self-association. (A and B) Stable transgenic, native promoter-driven 1×HA-RBA1 coimmunoprecipitates with Pf0-1–delivered T7-HopBA1 in Arabidopsis (A) and, when transiently coexpressed as 35S-driven 3xHA-RBA1, in N. benthamiana (B). Arrowheads indicate the RBA1 size shift associated with activity. (C) The RBA1 mobility shift is removed following treatment with protein phosphatase. (D) RBA1 self-association mutants are unable to coimmunoprecipitate HopBA1 when transiently coexpressed in N. benthamiana. (E) HopBA1 loss-of-response mutants partially lose association with RBA1 when transiently expressed in N. benthamiana. (F) RBA1 self-association is enhanced in the presence of WT Myc-HopBA1 but not HopBA1 H56F. See also Figs. S3–S5.