Fig. S4.

Phenotypes of RBA1 mutants (data related to Figs. 3 and 5). Mutations were made in residues predicted to affect either L6- or RPS4-type dimer interfaces. (A) Summary of transient N. benthamiana-Agrobacterium assays. Red outlines indicate nonresponsive mutants with phenotypes similar to the EV control. Yellow outlines indicate weak, partial loss-of-function phenotypes. (B) Identification of mutants producing the leaf phenotypes in A. Red boxes indicate strong loss-of-function phenotypes; orange boxes have partial loss-of-function phenotypes. Images were taken 5–7 d postinoculation. (C) Y2H assay. (D) Identification of mutants in the Y2H assay in C. (E) Western blot of Y2H.