Table 1.
Summary ofRBA1 mutant phenotypes
| Genotype | Putative interface | Cell death | Y2H self | Co-IP self | Co-IP HopBA1 |
| WT | Na | + | + | + | + |
| R26A | Na | + | + | Nt | Nt |
| G28A | RPS4 | + | + | Nt | Nt |
| G28R | RPS4 | − | + | Nt | Nt |
| S31A | RPS4 | + | + | + | + |
| H32A | RPS4 | − | + | − | − |
| S31A/H32A | RPS4 | − | − | − | Nt |
| D35K | RPS4 | ± | Nt | Nt | Nt |
| E38A | RPS4 | + | Nt | Nt | Nt |
| E38K | RPS4 | + | Nt | Nt | Nt |
| R39A | RPS4 | + | Nt | Nt | Nt |
| R39E | RPS4 | ± | Nt | Nt | Nt |
| L56A | Na | + | + | Nt | Nt |
| R109D | L6 | + | + | Nt | Nt |
| R111D | L6 | + | + | Nt | Nt |
| S114A | L6 | + | Nt | Nt | Nt |
| K149E | L6 | − | + | − | − |
| M150K | L6 | + | + | Nt | Nt |
| G151R | L6 | − | − | − | − |
| D156R | L6 | + | + | Nt | Nt |
| R158A | L6 | + | Nt | Nt | Nt |
| R158D | L6 | + | + | Nt | Nt |
| R158E | L6 | + | + | Nt | Nt |
| E160A | RPS4 | − | Nt | Nt | Nt |
| E160K | RPS4 | − | Nt | Nt | Nt |
| A161D | RPS4 | − | Nt | Nt | Nt |
| A161L | RPS4 | +/− | Nt | Nt | Nt |
| D162K | L6 | + | + | Nt | Nt |
| K165E | L6 | + | + | Nt | Nt |
| E166K | L6 | + | + | Nt | Nt |
| K169E | L6 | + | + | Nt | Nt |
| E170K | L6 | + | + | Nt | Nt |
| R173E | L6 | + | + | Nt | Nt |
“Putative interface” indicates that the residue lies in a hypothetical RPS4- or L6-like dimer interface. Data for cell death and Y2H self-association are from Fig. S4. Data for co-IP self are from Fig. 3. Data for co-IP HopBA1 are from Fig. 5. Co-ip, coimmunoprecipitation; Na, mutations not in predicted RPS4- or L6-type interfaces; Nt, not tested.