Fig. 6.

The TatB F13Y substitution promotes constitutive oligomerization of TatA in vivo. (A) Fluorescence images of TatA–YFP in representative cells of strains AyBCE or AyBC_F94QE (encoding the TatC F94Q substitution in chromosomal tatC) in the presence (pSUAmiA) or absence of plasmid-encoded wild-type AmiA, as indicated. Production of AmiA was induced by addition of 1 mM IPTG to mid-log cell cultures 45 min before harvesting. (B) Fluorescence images of TatA–YFP in representative cells of strains AyB_F13YCE (encoding the TatB F13Y substitution in chromosomal tatB) and AyBC_F94QE (encoding the TatB F13Y substitution in chromosomal tatB, alongside the TatC F94Q substitution in chromosomal tatC). (Scale bars, 1 μm.)