Fig. 4.

TIPARP binds to SINV RNA and induces SINV RNA degradation. (A) Wild-type MEFs stably expressing the indicated FLAG-tagged proteins were infected with SINV (MOI = 0.1) for 24 h. The levels of viral E2 protein, FLAG-tagged protein, and β-actin in cell lysates were determined by immunoblotting analysis. (B) Wild-type MEFs stably expressing the indicated expression plasmids were infected with SINV for 24 h. The levels of SINV RNA binding to the indicated FLAG-tagged proteins were measured by RNA immunoprecipitation, coupled with quantitative reverse transcription-PCR. (C) U373-CD14 cells were transiently transfected with the indicated expression plasmids. Cell lysates were subjected to immunoprecipitation with anti-FLAG antibody and to immunoblot analysis with anti-FLAG and anti-Myc antibodies. The experiments were performed three times, and representative data are shown (means ± SD of three independent samples). *P < 0.05.