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. 2017 Feb 17;114(10):2681–2686. doi: 10.1073/pnas.1621508114

Fig. S2.

Fig. S2.

Tiparp−/− dendritic cells respond normally to TLR ligands. (A) Flow cytometric analysis of splenoctyes from Tiparp+/+ and Tiparp−/− mice. Cell surface expression of the indicated maker proteins was measured. (B) ELISA of CXCL10, IL-6, and TNF in culture supernatants from primary Tiparp+/+ and Tiparp−/− granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cells stimulated with macrophage-activating lipopeptide-2 (MALP-2) (TLR2 ligand, 10 ng/mL), lipopolysaccharide (LPS) (TLR4 ligand, 100 ng/mL), R848 (TLR7 ligand, 10 μg/mL), or ODN 1668 (TLR9 ligand, 1 μM) for 24 h. (C) ELISA of IFN-α and IFN-β in culture supernatants from primary Tiparp+/+ and Tiparp−/− FMS-like tyrosine kinase 3 ligand-induced bone marrow-derived dendritic cells stimulated with ODN 1585 (TLR9 ligand, 5 μM) for 24 h. Experiments were performed three times, and representative data are shown (means ± SD of three independent samples).