Fig. S6.

MCL1 limits cytoplasmic accumulation of TIPARP. (A) Wild-type MEFs stably expressing TIPARP-FLAG with BCL2, BCLW, BCLXL, or MCL1 protein were infected with SINV (MOI = 5) for 12 h. Fixed samples were subjected to immunocytochemistry analysis of FLAG-tagged protein and Hoechst 33342 staining of genomic DNA. Frequencies of MEFs with cytoplasmic accumulation of TIPARP were determined. (B) Wild-type MEFs were infected with SINV (MOI = 0.5) for 12 h. Levels of viral protein, MCL1 protein, and β-actin in cell lysates were measured by immunoblotting analysis. (Scale bar, 20 μm.) Experiments were performed three times, and representative data are shown (means ± SD of three independent samples). *P < 0.05.