Fig. S7.

Rescue of tan1 mutant phenotype using multiple mD-TAN1–YFP lines. Maize epidermal cells from tan1 mutants expressing an individual mD-TAN1–YFP are represented in A–C. Cell walls are stained with propidium iodide (green). Brackets indicate the localization of mD-TAN1–YFP (magenta). Maize plants were grown for 28 d: siblings segregating wild-type and tan1 locus with mD-TAN1–YFP events were measured to assess rescue. Area measurements for each event and genotype are shown for leaf 5 (D), leaf 7 (E), and leaf 8 (F). Measurements for all leaves show that plants which are tan1 mD-TAN1 are not statistically different from wild-type indicating a rescue of the tan1 mutant dwarf phenotype (KS test, P > 0.1732, for all events). (Scale bar, 10 μm.)