Fig. 1.

Identification of natural and synthetic ligands of Mincle. (A) Chemical structures of TDM, TDB, GlcC14C18, ManC14C18, GlcC16, and GlcC4C12. (B–E) EC₅₀ values determined from the activation curves of HEK-hMincle (B and D) and HEK-mMincle (C and E) after incubation with mycobacterial natural compounds (B and C) or synthetic analogs (D and E). EC₅₀ data are presented as the mean ± SEM calculated from at least three independent experiments (SI Appendix, Fig. S2). All of the compound structures are described in SI Appendix, Fig. S1.