Fig. 6.

In vivo secretagogin silencing decreases DCX expression and slows neuroblast migration in the RMS. (A–A₂) Secretagogin (A₁ and A_1′) but not scrambled siRNA (A and A′) reduced DCX expression locally (arrowheads in A and A₁). This effect is independent of olfactory bulbectomy (Fig. S4). Note the reduced secretagogin expression upon using specific (A_1′) but not scrambled (A′) siRNA and compared with distal RMS free of silencing effect (A₂). (B–B₂) The density of BrdU⁺ cells decreased rostral but increased caudal to the silencing site (B₂). P < 0.05, Student’s t test. Images in A, A₁, B, and B₁ were acquired using the tile-and-stitch function. (Scale bars: 200 µm in A, A₁, B, and B₁; 25 µm in A′ and A_1′; 15 µm in B′–B_1′′′.)