Table S1.
Two-factorial ANOVA and post hoc analysis of in vivo analysis in rats
Effect | SS | Degree of freedom | MS | F | P | |||
Distal 1 RMS | ||||||||
Intercept | 15,620.13 | 1 | 15,620.13 | 1,360.608 | 0.000000 | |||
Treatment | 1,794.45 | 1 | 1,794.45 | 156.308 | 0.000000 | |||
Location | 451.78 | 1 | 451.78 | 39.353 | 0.000011 | |||
Treatment*location | 12.05 | 1 | 12.05 | 1.050 | 0.320806 | |||
Error | 183.68 | 16 | 11.48 | |||||
Cell no. | Bonferroni test; variable sqrtr Scgn+ cell number | |||||||
Probabilities for post hoc tests | ||||||||
Error: between MS = 11.48, df = 16 | ||||||||
Treatment | Location | [1] (24.003) | [2] (12.945) | [3] (41.395) | [4] (33.442) | |||
1 | Control | Marginal | 0.000569 | 0.000003 | 0.002658 | |||
2 | Control | Axis | 0.000569 | 0.000000 | 0.000000 | |||
3 | Bulbectomy | Marginal | 0.000003 | 0.000000 | 0.011379 | |||
4 | Bulbectomy | Axis | 0.002658 | 0.000000 | 0.011379 | |||
Distal 2 RMS | ||||||||
Intercept | 13,675.81 | 1 | 13,675.81 | 1,514.256 | 0.000000 | |||
Treatment | 1,595.27 | 1 | 1,595.27 | 176.636 | 0.000000 | |||
Location | 426.40 | 1 | 426.40 | 47.213 | 0.000004 | |||
Treatment*location | 12.56 | 1 | 12.56 | 1.391 | 0.255439 | |||
Error | 144.50 | 16 | 9.03 | |||||
Cell no. | Bonferroni test; variable sqrtr Scgn+ cell number | |||||||
Probabilities for post hoc tests | ||||||||
Error: between MS = 9.03, df = 16 | ||||||||
Treatment | Location | [1] (22.628) | [2] (11.808) | [3] (38.905) | [4] (31.256) | |||
1 | Control | Marginal | 0.000200 | 0.000001 | 0.002012 | |||
2 | Control | Axis | 0.000200 | 0.000000 | 0.000000 | |||
3 | Bulbectomy | Marginal | 0.000001 | 0.000000 | 0.005880 | |||
4 | Bulbectomy | Axis | 0.002012 | 0.000000 | 0.005880 | |||
Distal 3 RMS | ||||||||
Intercept | 10,765.17 | 1 | 10,765.17 | 1,254.685 | 0.000000 | |||
Treatment | 985.11 | 1 | 985.11 | 114.815 | 0.000000 | |||
Location | 446.42 | 1 | 446.42 | 52.031 | 0.000002 | |||
Treatment*location | 7.82 | 1 | 7.82 | 0.911 | 0.353953 | |||
Error | 137.28 | 16 | 8.58 | |||||
Cell no. | Bonferroni test; variable sqrtr Scgn+ cell number | |||||||
Probabilities for post hoc tests | ||||||||
Error: between MS = 8.58, df = 16 | ||||||||
Treatment | Location | [1] (21.532) | [2] (10.832) | [3] (34.318) | [4] (26.119) | |||
1 | Control | Marginal | 0.000170 | 0.000021 | 0.148985 | |||
2 | Control | Axis | 0.000170 | 0.000000 | 0.000002 | |||
3 | Bulbectomy | Marginal | 0.000021 | 0.000000 | 0.002545 | |||
4 | Bulbectomy | Axis | 0.148985 | 0.000002 | 0.002545 |
Quantitative analysis of secretagogin+ neurons in rats after bulbectomy in the axial and marginal regions of the RMS. Treatment: bulbectomy vs. control. Location: marginal vs. axial. MS, mean of squares; SS, sum of squares; variable sqrtr, variable under square root transformation.