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. 2017 Feb 21;114(10):E2006–E2015. doi: 10.1073/pnas.1700662114

Table S3.

Possible secretagogin-interacting molecules

Identified proteins, n = 64 Accession no. Massin kDA No. NTS IgG, minimum twofold less than the no. of NTS Scgn Quantitative value (normalized total spectra) Exclusive unique peptide counts Protein identification probability, % Percent coverage
Description Gene name Ctrl, no Ca Ctrl, Ca OBX, no Ca OBX, Ca Ctrl, no Ca Ctrl, Ca OBX, no Ca OBX, Ca Ctrl, no Ca Ctrl, Ca OBX, no Ca OBX, Ca Ctrl, no Ca Ctrl, Ca OBX, no Ca OBX, Ca Ctrl, no Ca Ctrl, Ca OBX, no Ca OBX, Ca
IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn IgG Scgn
14-3-3 protein zeta/delta Ywhaz A0A0G2JV65_RAT 29 * 6 2 7 14 12 11 10 10 4 2 4 5 5 4 3 3 100 100 100 100 100 100 100 100 19 9 19 26 26 16 13 13
2,4-dienoyl-CoA reductase, mitochondrial Decr1 DECR_RAT (+1) 36 * 0 0 0 0 0 3 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 7 0 0
60S ribosomal protein L23 Rpl23 RL23_RAT 15 * 1 2 0 0 2 3 0 0 1 2 0 0 2 2 0 0 100 100 0 0 100 100 0 0 14 25 0 0 21 25 0 0
Actn1 protein Actn1 Q6GMN8_RAT 103 * 28 6 2 6 28 7 5 10 15 2 1 4 16 4 3 8 100 100 100 100 100 100 100 100 21 3 2 5 22 6 4 11
ADP/ATP translocase 1 Slc25a4 ADT1_RAT 33 * 1 2 1 2 6 6 2 4 1 2 1 2 4 3 2 2 100 100 100 100 100 100 100 100 4 7 3 8 14 11 8 7
Annexin A5 Anxa5 ANXA5_RAT (+1) 36 * 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 0 0 98 0 0 5 0 100 0 0 0 0 0 0 0 10
ATP synthase subunit alpha, mitochondrial Atp5a1 ATPA_RAT 60 * 0 0 4 8 0 1 1 4 0 0 2 3 0 1 1 3 0 0 100 100 0 100 100 100 0 0 5 6 0 2 3 7
ATP synthase subunit O, mitochondrial Atp5o ATPO_RAT 23 * 7 2 4 4 8 7 1 4 5 1 3 3 5 5 1 2 100 100 100 100 100 100 98 100 33 10 16 19 34 34 8 17
ATP-dependent 6-phosphofructokinase Pfkm A0A0G2KBC7_RAT 94 * 3 0 10 6 0 0 4 9 2 0 6 4 0 0 2 6 100 83 100 100 0 100 100 100 3 0 8 5 0 0 3 9
ATP-dependent 6-phosphofructokinase, platelet type Pfkp PFKAP_RAT 86 * 1 0 8 9 0 3 6 9 1 0 3 5 0 2 4 6 100 0 100 100 38 100 100 100 2 0 6 10 0 4 9 11
ATP-dependent RNA helicase DDX1 Ddx1 DDX1_RAT 82 * 7 2 1 0 1 2 2 1 3 2 1 0 1 2 1 1 100 100 100 98 100 100 99 98 5 3 2 0 2 3 2 2
Beta-adducin Add2 F8WFS9_RAT 81 * 8 1 4 1 7 5 2 9 3 1 3 1 4 3 2 4 100 90 100 100 100 100 100 100 6 1 6 1 7 6 4 7
Calcium/calmodulin-dependent protein kinase type II subunit gamma Camk2g F1LMC3_RAT (+1) 59 * 60 33 0 43 35 33 64 52 3 1 0 2 3 3 3 2 100 91 0 100 100 100 100 100 18 14 0 16 18 18 20 16
Cofilin-1 Cfl1 COF1_RAT 19 * 1 0 1 1 5 3 1 3 1 0 1 1 4 2 1 2 100 0 98 98 100 100 100 100 12 0 7 7 30 17 7 19
Complement C1q subcomponent subunit C C1qc C1QC_RAT 26 * * * 0 5 0 4 0 3 0 4 0 2 0 2 0 1 0 2 12 100 98 100 46 100 99 100 0 8 0 8 0 4 0 8
Complement C3 C3 M0RBF1_RAT 186 * * * * 12 61 10 54 7 45 6 29 5 8 3 8 4 8 2 7 100 100 100 100 100 100 100 100 3 5 2 5 3 5 1 4
Coronin Coro1b G3V940_RAT 54 * 1 0 0 2 1 2 1 1 1 0 0 2 1 1 1 1 100 48 0 100 100 100 99 100 2 0 0 4 2 2 2 2
Coronin Coro2a A0A0G2QC56_RAT 62 * * 1 2 0 1 6 2 1 7 1 2 0 1 4 2 1 4 99 100 12 100 100 100 100 100 2 4 0 2 10 4 2 8
Coronin Coro2b F1LMV9_RAT 55 * * 5 2 1 4 9 7 3 10 3 2 1 2 4 3 1 2 100 100 100 100 100 100 100 100 8 5 3 5 11 7 3 5
Coronin-1A Coro1a COR1A_RAT 51 * 0 0 0 3 2 1 3 4 0 0 0 2 2 1 2 3 54 0 0 100 100 100 100 100 0 0 0 5 4 2 4 6
Cortactin-binding protein 2 Cttnbp2 sp|Q2IBD4|CTTB2_RAT 179 * 6 0 0 0 0 3 0 0 4 0 0 0 0 3 0 0 100 67 0 94 100 100 0 96 4 0 0 0 0 2 0 0
Creatine kinase B-type Ckb KCRB_RAT 43 * 0 0 1 3 0 0 0 0 0 0 1 2 0 0 0 0 0 0 99 100 0 6 0 0 0 0 3 6 0 0 0 0
Creatine kinase, mitochondrial 1, ubiquitous Ckmt1b Q5BJT9_RAT 47 * * 14 11 0 2 6 12 0 1 4 3 0 2 3 4 0 1 100 100 68 100 100 100 8 99 13 11 0 9 11 13 0 5
Elongation factor 1-alpha 1 Eef1a1 EF1A1_RAT 50 * 8 10 4 3 10 12 2 6 4 3 3 1 4 5 2 4 100 100 100 100 100 100 100 100 13 7 6 2 13 11 4 9
F-actin-capping protein subunit beta Capzb CAPZB_RAT 31 * 14 2 3 8 19 12 9 11 6 2 2 4 7 7 3 5 100 100 100 100 100 100 100 100 17 9 9 19 25 28 14 22
Fascin Fscn1 FSCN1_RAT 54 * * 13 11 0 3 8 10 3 10 8 5 0 2 7 6 2 6 100 100 10 100 100 100 100 100 18 10 0 4 16 14 5 14
Fc of IgG, low affinity IIIa, receptor Fcgr3a A0A0B4J2J1_RAT (+1) 28 * 0 2 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 100 0 27 0 10 0 97 0 10 0 0 0 0 0 0
Flightless I homolog (Drosophila) Flii Q5RKI5_RAT 145 * 16 5 3 5 22 15 1 11 9 2 2 5 9 7 1 6 100 100 100 100 100 100 100 100 9 2 2 4 9 7 1 6
Fructose-bisphosphate aldolase A Aldoa ALDOA_RAT 39 * 4 1 4 1 0 2 0 0 3 1 2 1 0 2 0 0 100 91 100 97 6 100 0 0 13 2 6 4 0 7 0 0
Glutamate dehydrogenase 1, mitochondrial Glud1 DHE3_RAT 61 * * 9 6 0 4 11 25 0 2 5 3 0 2 8 14 0 1 100 100 23 100 100 100 16 100 9 5 0 3 17 31 0 1
Hemoglobin alpha, adult chain 2 Hba1 B1H216_RAT (+1) 15 * * 2 6 5 8 2 3 2 6 2 3 4 5 2 3 2 3 100 100 100 100 100 100 100 100 22 26 46 49 20 33 15 26
Hemoglobin subunit beta-1 Hbb HBB1_RAT 16 * * 7 9 7 6 3 7 1 9 5 5 4 4 2 4 1 4 100 100 100 100 100 100 100 100 41 44 30 28 14 35 8 28
Histone H2A Hist1h2af Q6I8Q6_RAT 14 * 3 0 0 2 0 0 1 2 2 0 0 2 0 0 1 1 100 0 0 100 31 16 100 97 22 0 0 22 0 0 7 15
Histone H4 Hist1h4b sp|P62804|H4_RAT 11 * 0 0 0 12 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 39 0 0 0 0
Ig gamma-2B chain C region Igh-1a IGG2B_RAT 36 * 2 4 0 2 1 2 0 1 1 1 0 1 1 2 0 1 100 100 0 100 100 100 13 100 3 3 0 3 3 9 0 3
Ig kappa chain C region, A allele N/A KACA_RAT 12 * * 0 12 0 2 2 6 0 0 0 3 0 1 1 3 0 0 0 100 0 99 95 100 0 94 0 36 0 12 12 36 0 0
Junction plakoglobin Jup PLAK_RAT 82 * 1 0 0 7 1 0 0 0 1 0 0 5 1 0 0 0 98 57 0 100 95 0 0 0 2 0 0 9 2 0 0 0
Microtubule-associated protein Mapt D3ZKD9_RAT 39 * 6 19 0 0 7 10 0 0 3 6 0 0 3 4 0 0 100 100 0 0 100 100 0 0 10 20 0 0 10 13 0 0
Myelin basic protein Mbp sp|P02688|MBP_RAT 22 * 73 134 1 7 70 71 7 4 7 8 1 5 7 7 5 3 100 100 99 100 100 100 100 100 38 41 5 28 38 38 28 17
Nonspecific serine/threonine protein kinase Cdc42bpb A0A0G2KB58_RAT 194 * 1 1 0 1 5 6 1 11 1 1 0 1 5 6 1 8 100 100 21 100 100 100 100 100 1 1 0 1 4 4 1 6
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), isoform CRA_a Ogt A0A0G2K3V4_RAT 117 * * 7 25 1 0 6 14 0 0 5 13 1 0 4 10 0 0 100 100 99 0 100 100 0 0 6 15 1 0 5 12 0 0
Peroxisomal multifunctional enzyme type 2 Hsd17b4 DHB4_RAT (+1) 79 * 4 4 0 0 1 3 0 3 2 3 0 0 1 2 0 1 100 100 57 0 100 100 0 100 4 5 0 0 2 3 0 2
Phosphorylase kinase, beta Phkb Q5RKH5_RAT 126 * 0 0 0 3 0 0 0 0 0 0 0 2 0 0 0 0 43 0 12 100 0 0 0 46 0 0 0 3 0 0 0 0
Protein Diras2 Diras2 D3ZHX3_RAT 22 * 0 1 0 1 0 2 0 1 0 1 0 1 0 2 0 1 0 100 0 96 0 100 0 97 0 6 0 6 0 12 0 6
Protein Dmxl2 Dmxl2 F1M3W5_RAT 341 * 3 0 3 4 1 1 1 5 2 0 3 2 1 1 1 4 100 12 100 100 100 100 100 100 1 0 1 1 1 1 1 2
Protein Golga3 Golga3 D3ZLD5_RAT 163 * * 0 0 1 9 0 0 0 7 0 0 1 4 0 0 0 5 6 0 97 100 0 0 0 100 0 0 1 4 0 0 0 5
Protein Hcfc1 Hcfc1 D3ZN95_RAT 209 * * 2 21 0 0 1 4 0 0 2 9 0 0 1 4 0 0 100 100 0 0 100 100 0 0 1 5 0 0 1 3 0 0
Protein LOC100362298 LOC100362298 D3ZM33_RAT 18 * 3 7 0 0 5 5 0 0 3 4 0 0 5 4 0 0 100 100 0 0 100 100 0 0 19 24 0 0 30 26 0 0
Protein rogdi homolog Rogdi ROGDI_RAT 32 * 0 0 1 1 0 2 0 4 0 0 1 1 0 1 0 3 41 61 99 100 32 100 30 100 0 0 5 7 0 7 0 12
Protein Sacs Sacs D4A1D3_RAT 521 * 3 1 1 2 6 4 2 14 2 1 1 2 4 4 2 8 100 99 100 100 100 100 100 100 0 0 0 0 1 1 1 2
Protein Slc25a12 Slc25a12 F1LX07_RAT 72 * 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 0 0 95 26 0 0 21 100 0 0 0 0 0 0 0 4
Protein Vps35 Vps35 G3V8A5_RAT 92 * 1 0 1 1 1 0 1 3 1 0 1 1 1 0 1 2 100 0 100 100 87 0 99 100 1 0 1 1 1 0 2 3
RAB10, member RAS oncogene family Rab10 Q5RKJ9_RAT 23 * 0 0 2 3 1 0 0 4 0 0 0 1 0 0 0 2 12 10 69 100 22 0 0 100 0 0 6 11 6 0 0 17
Rho guanine nucleotide exchange factor 11 Arhgef11 A0A0G2JZC6_RAT 174 * 2 0 0 0 1 2 0 0 2 0 0 0 1 2 0 0 100 26 17 0 100 100 11 86 1 0 0 0 1 1 0 0
Secretagogin Scgn F1LN07_RAT 32 * * * * 0 47 0 31 0 54 0 55 0 12 0 10 0 15 0 11 0 100 0 100 0 100 0 100 0 56 0 51 0 60 0 50
Septin-7 Sept7 D4A0F5_RAT 51 * 27 20 0 0 13 11 0 2 6 6 0 0 4 4 0 2 100 100 0 10 100 100 74 100 19 19 0 0 11 11 0 4
Stress-70 protein, mitochondrial Hspa9 GRP75_RAT 74 k * 1 2 3 3 6 1 4 6 1 2 3 3 5 1 4 5 100 100 100 100 100 100 100 100 2 4 6 6 9 2 7 10
Synapsin II, isoform CRA_a Syn2 G3V733_RAT (+1) 63 * * 37 81 1 0 24 48 5 2 4 6 1 0 1 5 1 1 100 100 100 25 100 100 100 100 14 15 3 0 8 17 6 3
Tenascin-R Tnr F1LQ63_RAT 150 * 0 0 14 0 0 0 0 2 0 0 4 0 0 0 0 2 0 0 100 97 0 0 97 100 0 0 4 0 0 0 0 2
Transgelin-3 Tagln3 TAGL3_RAT 23 * 30 22 0 6 46 57 8 12 11 8 0 3 12 12 6 8 100 100 0 100 100 100 100 100 63 41 0 17 71 71 32 41
Triosephosphate isomerase Tpi1 TPIS_RAT 27 * * 1 0 0 3 2 1 2 5 1 0 0 2 1 1 2 5 100 62 31 100 100 100 100 100 6 0 0 10 5 5 10 28
Tropomodulin-2 Tmod2 TMOD2_RAT 39 * 22 17 11 23 22 24 22 21 5 4 4 5 7 4 4 5 100 100 100 100 100 100 100 100 17 14 14 17 29 14 14 16
Tropomyosin alpha-3 chain Tpm3 sp|Q63610|TPM3_RAT 29 * 0 0 0 3 0 0 1 0 0 0 0 2 0 0 1 0 43 0 0 100 0 0 88 51 0 0 0 10 0 0 4 0
Unconventional myosin-Ib Myo1b D4A2X2_RAT (+1) 132 * 8 0 7 7 11 4 4 10 6 0 5 5 7 3 3 6 100 10 100 100 100 100 100 100 6 0 6 5 8 4 3 7

Asterisks indicate proteins identified with a minimum 99% probability, with a mininimum of two exclusive unique peptide counts, and with at least twofold higher normalized total spectra following immunoprecipitation. Taxonomic names for all: Rattus norvegicus. NTS, normalized total spectra.