Fig. 2.

Ability of embryonic body formation and staining of Tra1-81 in reprogrammed Hep3B cells by retrovirus-KOSM. HepG2 nontransduced cells were used as a negative control for KOSM transduction (A, scale bar=200 μm and B, scale bar=100 μm). HepG2 (C, G, K, and O), PLC (D, H, L, and P), Huh7 (E, I, M, and Q), and Hep3B (F, J, N, and R) were transduced with KOSM retroviral-vector (C–J, passage 1 and K–R, passage 2). Phase contrast (C–F and K–N) and Tra1-81 staining (G–J and O–R) are shown. Scale bar=100 μm. Embryonic body formation in Hep3B-iPC cells was demonstrated by culturing for 3 days (S, phase contrast; scale bar=200 μm).