Table 1.

Primer Sequences Used in qPCR Analysis

Gene	Primer sequence	Tm, °C
Oct4-F	5′-GATGTGGTCCGAGTGTGGTT-3′	60
Oct4-R	5′-AGCCTGGGGTACCAAAATGG-3′
Nanog-F	5′-AAGGCCTCAGCACCTACCTA-3′	60
Nanog-R	5′-TGCACCAGGTCTGAGTGTTC-3′
Sox2-F	5′-GCCCTGCAGTACAACTCCAT-3′	60
Sox2-R	5′-GACTTGACCACCGAACCCAT-3′
Foxa2-F	5′-ATTGCTGGTCGTTTGTTGTG-3′	60
Foxa2-R	5′-CCTCGGGCTCTGCATAGTAG-3′
Sox17-F	5′-GGCGCAGCAGAATCCAGA-3′	60
Sox17-R	5′-CCACGACTTGCCCAGCAT-3′
Goosecoid-F	5′-GAGGAGAAAGTGGAGGTCTGGTT-3′	60
Goosecoid-R	5′-CTCTGATGAGGACCGCTTCTG-3′
Msx1-F	5′-CGAGAGGACCCCGTGGATGCAGAG-3′	60
Msx1-R	5′-GGCGGCCATCTTCAGCTTCTCCAG-3′
Sox1-F	5′-GGAATGGGAGGACAGGATTT-3′	60
Sox1-R	5′-AACAGCCGGAGCAGAAGATA-3′
Map2-F	5′-CAGGTGGCGGACGTGTGAAAATTGAGAGTG-3′	60
Map2-R	5′-CACGCTGGATCTGCCTGGGGACTGTG-3′
GAPDH-F	5′-GGACTCATGACCACAGTCCATGCC-3′	60
GAPDH-R	5′-TCAGGGATGACCTTGCCCACAG-3′
Endo-Oct4-F	5′-GACAGGGGGAGGGGAGGAGCTAGG-3′	60
Endo-Oct4-R	5′-CTTCCCTCCAACCAGTTGCCCCAAAC-3′
Endo-Sox2-F	5′-GGGAAATGGGAGGGGTGCAAAAGAGG-3′	60
Endo-Sox2-R	5′-TTGCGTGAGTGTGGATGGGATTGGTG-3′
Endo-Klf4-F	5′-ACGATCGTGGCCCCGGAAAAGGACC-3′	60
Endo-Klf4-R	5′-TGATTGTAGTGCTTTCTGGCTGGGCTCC-3′
Endo-Myc-F	5′-GCGTCCTGGGAAGGGAGATCCGGAGC-3′	60
Endo-Myc-R	5′-TTGAGGGGCATCGTCGCCGGAGGCTG-3′
Exo-Oct4-F	5′-CAACGAGAGGATTTTGAGGCT-3′	60
Exo-Sox2-F	5′-TGCAGTAGAACTCCATGACCA-3′
Exo-Klf4-F	5′-TGCGGCAAAACCTACACAAAG-3′
Exo-Myc-F	5′-CAACAACCGAAATGCACCAGCCCCAG-3′
pMX-Tg-R	5′-TACAGGTGGGGTCTTTCATTC-3′
hGAPDH-F	5′-AACAGCCTCAAGATCATCAGC-3′	60
hGAPDH-R	5′-TTGGCAGGTTTTTCTAGACGG-3′

qPCR, real-time polymerase chain reaction; Tm, annealing temperature; F, forward; R, reverse.