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. 2016 Sep 10;7(50):81981–81994. doi: 10.18632/oncotarget.11962

Figure 2. LINC00520 is regulated by the PI3K pathway.

Figure 2

a. Schematic of MCF10A system used to identify PI3K regulated genes involved in transformation. b. Heat map of gene expression values from Src-transformed MCF10A cells for genes that increased (left) or decreased (right) by two fold in the mutant PIK3CA-transformed MCF10A cells. Quantitative RT-PCR, showing relative expression of LINC00520 in: c. MCF10A cells expressing wild-type PIK3CA (WT) compared to mutant PIK3CA H1047R, d. Isogenic MDA-MB-231 cells expressing wild-type PIK3CA (WT), and mutant PIK3CA H1047R respectively and e. shRNA-mediated knockdown of PIK3CA in SUM159-PT cells. Transcript levels were normalized to GAPDH. Data represents average of three independent experiments ± the SEM. Statistical significance was determined using paired Student's t-test, * p < 0.05, ** p < 0.01, ***p < 0.001.