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. 2016 Sep 10;7(50):81981–81994. doi: 10.18632/oncotarget.11962

Figure 5. Depletion of LINC00520 affects the integrity of invasive protrusions.

Figure 5

a. Quantitative RT-PCR validation of silencing of LINC00520 in MCF10DCIS cells. Transcript levels were normalized to GAPDH and fold change was calculated relative to scrambled shRNA #1. b. Representative phase-contrast images of MCF10DCIS acini infected with lentiviral scrambled or LINC00520 shRNA grown in 3D Matrigel. Images displayed were taken on day 5. c. Morphological effects of silencing of LINC00520 in MCF10DCIS cells grown in (Top) 2D, scale bar = 100μm and (Bottom) in 3D Matrigel cultures. For 3D, images shown were taken at day 5, scale bar = 500μm.