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. 2016 Jul 9;7(50):82213–82227. doi: 10.18632/oncotarget.10511

Figure 1. EBV BARF1 protein was mainly secreted and promoted cell proliferation.

Figure 1

(A) BARF1 mRNA was detected in SNU601 BARF1 and SNU216 BARF1 cells (stable transfection with BARF1) and in naturally Epstein-Barr virus (EBV)-infected stomach cancer cells (SNU719 and YCCEL1), whereas it was undetectable in SNU601 mock cells and SNU216 mock cells. (B) As seen by fluorescence microscopy, BARF1 protein was hardly observed in SNU601 BARF1 cells and was weakly detected in SNU216 BARF1, SNU719 and YCCEL1 cells, whereas BARF1 protein accumulated in cells that were treated with Brefeldin A. BARF1 antibody (MAb 6F4) was labeled in red, and nuclei were counterstained with DAPI (blue). (C) Cell proliferation was higher in SNU601 BARF1 and SNU216 BARF1 cells than in SNU601 mock cells and SNU216 mock cells, respectively, and lower in YCCEL1 cells transfected with BARF1-specific siRNA (siBARF1) than in YCCEL1 cells transfected with scrambled siRNA (siSCR) (*P < 0.05). All experiments were performed in triplicate.